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. 2023 Aug 21;22(10):e13967. doi: 10.1111/acel.13967

FIGURE 2.

FIGURE 2

SIRT1 and SIRT2 have opposite effects on cell survival under Aβ42 challenge. (a, b) Western blotting and quantification of the protein expression level of SIRT1 and SIRT2 upon Aβ42 stimulus (10 μΜ) in HT22 cells (n = 3). (c) Cell viability was measured by the CCK8 assay. (d) Lactate dehydrogenase levels (LDH) were measured in the supernatants of SH‐SY5Y cells (n = 4). ** indicates p < 0.01 compared with empty vector + Aβ group; ## indicates p < 0.01 compared with empty vector + DMSO group. (e, f) Fluorescence‐activated cell sorting (FACS) and statistical analysis showing apoptosis of indicated SH‐SY5Y cells (n = 3). Data are means ± SEM. Asterisk depicts p values (*p < 0.05, **p < 0.01) as observed by Student's t test (b–d) or one‐way ANOVA with Tukey's multiple comparison test (f), as applicable.