TABLE 2.
Activity against gram-negative bacteriaa
| Organism | MIC (μg/ml) of the following compounds in the presence of NaCl at the indicated concn:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| LL-37
|
HNP-1
|
PG-1
|
Gentamicin
|
|||||
| 0 mM | 100 mM | 0 mM | 100 mM | 0 mM | 100 mM | 0 mM | 100 mM | |
| E. coli ML-35p | 0.6 | 7.6 | 0.7 | 18.4 | 0.5 | 0.7 | 0.02 | 0.08 |
| E. coli ATCC 9637 | 0.1 | 2.1 | 1.8 | 67.1 | 0.2 | 0.5 | >250 | >250 |
| E. coli ATCC 11775 | 1.9 | 2.7 | 2.2 | >250 | 0.2 | 0.6 | 0.03 | 0.09 |
| E. coli MCR 106 | 1.6 | 2.1 | 3.7 | >79.1 | 0.2 | 0.4 | 0.02 | 0.06 |
| S. typhimurium 7953s | 0.4 | 3.6 | 8.4 | >250 | 0.1 | 0.4 | 0.08 | 0.7 |
| S. typhimurium 14028s | 1.2 | 2.9 | >250 | >250 | 1.8 | 0.9 | 0.03 | 0.3 |
| P. aeruginosa MR 3007 | 4.7 | 3.8 | >250 | >250 | 0.8 | 0.7 | 65.6 | >79.1 |
| P. aeruginosa MR 53647-1 CF | 2.5 | 3.7 | >250 | >250 | 0.3 | 0.9 | 4.3 | 5.8 |
| P. aeruginosa AML 654 (mucoid) | 5.7 | 3.6 | >250 | >250 | 0.5 | 0.7 | 6.5 | 25.1 |
| P. aeruginosa CL79 | 1.4 | 0.9 | >250 | >250 | 0.3 | 0.4 | 0.07 | 0.7 |
| P. aeruginosa SBI-N | 1.3 | 4.0 | >250 | >250 | 0.5 | 0.7 | 0.15 | 0.2 |
| B. cepacia 96-11 | >79.1 | >250 | >250 | >250 | 17.0 | 53.9 | 19.4 | >79.1 |
| B. cepacia ATCC 25416 | >79.1 | >250 | >250 | >250 | 7.4 | 22.5 | 25.5 | >79.1 |
| S. maltophilia 411 A-15 | 1.9 | 5.1 | 4.3 | >250 | 0.2 | 0.4 | >79.1 | >250 |
| P. mirabilis ATCC 7002 | 5.7 | >25.0 | >250 | >250 | 0.6 | 1.4 | 0.12 | 0.7 |
| P. vulgaris ATCC 13315 | 2.5 | 5.4 | >79.1 | >250 | 0.2 | 0.6 | 0.07 | 0.08 |
a
Radial diffusion assays were performed with the standard low-salt underlays and with otherwise identical underlays that also contained 100 mM NaCl. MICs correspond to the x intercepts of plots such as those illustrated in Fig. 2. The molecular mass of gentamicin C1 is 477. The masses of the peptides are as follows: LL-37, 4,493; HNP-1, 3,442; PG-1, 2,056.