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. Author manuscript; available in PMC: 2023 Oct 16.
Published in final edited form as: Cell Rep. 2023 Jul 29;42(8):112911. doi: 10.1016/j.celrep.2023.112911

Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1.

Figure 2.

WT and TCF1 KO OT1 cells were co-transferred into WT C57BL/6 recipients that were then vaccinated or challenged with LM-OVA.

The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry.

(A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells.

(B) The number of total WT and KO OT1 cells split into CD127+ and CD127− subsets.

(C) The ratio of the number of WT cells divided by the number of KO cells in (B).

(D) The gMFI of CD127 in WT and KO OT1 cells.

(E) Representative contour plots for CD127 versus TCF1 staining.

(F) The number of total WT and KO OT1 cells split into CD127+ and CD127− subsets.

(G) The ratio of the number of WT cells divided by the number of KO cells in (F).

(H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge.

Data shown are mean ± SEM; n ≥ 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.