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. Author manuscript; available in PMC: 2023 Oct 16.
Published in final edited form as: Cell Rep. 2023 Jul 29;42(8):112911. doi: 10.1016/j.celrep.2023.112911

Figure 7. CD8 responses to mRNA-LNP vaccination are also T-bet and FOXO1 dependent.

Figure 7.

WT (CD45.1/1) and KO (CD45.1/2) OT1 cells were co-transferred into CD45.2/2 C57BL/6 recipients. One day later, the mice were vaccinated with OVA-encoding mRNA-LNPs as described in the STAR Methods. On day 10 post vaccination, the spleens were harvested, and the WT:KO ratio and phenotype of the responding OT1s were was analyzed by flow cytometry.

(A) Representative dot plots of spleen cells, gated only on the transferred T cells, in mice co-transferred with WT:FOXO KO (left) or WT:T-bet KO (right) cells. Numbers represent percentages of WT or KO T cells of total transferred T cells in each panel.

(B) Quantification of WT and KO T cell numbers from (A).

(C) Ratio of WT:KO T cells in each recipient.

Data shown are mean ± SEM; representative of two experiments with n = 8–10 mice per group. Significance was defined by paired t test, where *p < 0.05, **p < 0.01.