Fig. 2.

Effect of autophagy on antioxidase system under oxidative stress

(A-B) ROS determined by DCFH-DA. Two hours after pretreatment with Rapamycin or 3-MA, HLECs were irradiated with two doses of UVB. After 24 ​h, intracellular ROS levels were determined using a commercial kit. (A) Observation under a fluorescence microscope, and (B) detection by a fluorescence microplate reader. (C) Western blot was used to detect the levels of p62, LC3I, and LC3II protein 24 ​h after two does of UVB irradiation to HLECs pretreated with Rapamycin or 3-MA. (D) p62, LC3I and LC3II protein level. (E) Expression level of HO-1, GST and NQO1 expression level detected by qPCR (mean ​± ​s.d.; n ​= ​3, ∗P ​< ​0.05, ∗∗P ​< ​0.01) 24 ​h after two doses of UVB irradiation to HLECs pretreated with Rapamycin or 3-MA.