Fig. 3.

Apoptosis assay of differently treated HLECs under two doses of UVB irradiation

(A-B) Annexin V/PI staining detected by flow cytometry. (C–D) Counting apoptotic cells and autophagosomes by Transmission Electron Microcopy. 100 ​cells were randomly selected and the apoptotic cells (thick arrows) and the number of autophagosomes per cell (thin arrows) were counted. (E) Two hours after pretreatment with Rapamycin or 3-MA, HLECs was irradiated with two doses of UVB. After 24 ​h, Western blot was used to detect the levels of Bcl-2 and Bax protein. (F) Bcl-2 and Bax protein level (mean ​± ​s.d.; n ​= ​3, ∗P ​< ​0.05,∗∗P < ​0.01,∗∗∗P ​< ​0.001).