Skip to main content
. Author manuscript; available in PMC: 2024 Oct 9.
Published in final edited form as: Curr Biol. 2023 Sep 13;33(19):4174–4186.e7. doi: 10.1016/j.cub.2023.08.046

Key resources table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
Ethyl Methanesulfonate Sigma-Aldrich M0880; CAS: 62–50-0
Sodium Azide Sigma-Aldrich S2002; CAS: 26628–22-8
Experimental models: Organisms/strains
hmnIs82 [grl-18pro:GFP] II Fung et al., 202019 CHB3829
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V This paper CHB3843
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; hmnEx2172 [prab-3:fem-3-mCherry] This paper CHB3925
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; hmnEx2193 [mir-228pro:fem-3; unc-122pro:RFP] This paper CHB3900
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; hmnEx2215 [col-56pro:fem-3; unc-122pro:RFP] This paper CHB4032
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; hmnEx2208 [prab-3:tra-2-mCherry] This paper CHB4030
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; hmnEx2204 [mir-228pro:tra-2; unc-122pro:GFP] This paper CHB3926
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; hmnEx2211 [col-56pro:tra-2; unc-122pro:RFP] This paper CHB4031
nIs131 [pkd-2:GFP] I; hmnIs82 [grl-18pro:GFP] II;
him-5(e1490) V		 This paper CHB4298
nIs131 [pkd-2:GFP] I; hmnIs82 [grl-18pro:GFP] II;
him-5(e1490) V; ceh-30(n3714) X		 This paper CHB4285
nIs131 [pkd-2:GFP] I; hmnIs82 [grl-18pro:GFP] II;
him-5(e1490) V; ceh-30(n4289) X		 This paper CHB4338
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V;
nfya-1(ok1174) X		 This paper CHB4185
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V;
nfya-1(ok1174) X; hmnEx4304 [nfya-1pro:nfya-1-cDNA;
unc-122pro:RFP]		 This paper CHB4245
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V;
nfya-1(ok1174) X; hmnEx2329 [col-56pro:nfya-1-cDNA; unc-122pro:RFP]		 This paper CHB4303
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V;
nfya-1(ok1174) X; hmnEx2535 [rab-3pro:nfya-1-cDNA;
unc-122pro:RFP]		 This paper CHB4830
nfyb-1(cu13) II; him-5(e1490) V;
hmnIs78 [grl-18pro:GFP] X		 This paper CHB4281
nfyc-1(tm4541) II; him-5(e1490) V;
hmnIs78 [grl-18pro:GFP] X		 This paper CHB4603
hmnIs82 [grl-18pro:GFP] mab-3(mu15) II;
him-5(e1490) V		 This paper CHB3845
hmnIs82 [grl-18pro:GFP] mab-3(mu15) II; him-5(e1490) V; nfya-1(ok1174) X This paper CHB4220
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV; hmnEx2400 [grl-18pro:sfGFP:grl-18-gDNA;
rol-6(su1006)]		 This paper CHB4467
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV; hmnEx2486 [grl-18pro:grl-18-gDNA:sfGFP;
rol-6(su1006)]		 This paper CHB4642
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV; hmnEx2398 [grl-18pro:sfGFP:grl-18-gDNA(352–445); rol-6(su1006)] This paper CHB4465
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV; hmnEx2394 [grl-18pro:sfGFP:grl-18-gDNA(31–351);
rol-6(su1006)]		 This paper CHB4461
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV;
grl-18(syb6299 [sfGFP:grl-18]) V		 This paper CHB4641
him-5(e1490) V; hmnEx2422 [pkd-2pro:GFP;
dat-1pro:mApple; rol-6(su1006)]		 This paper CHB4519
him-5(e1490) V; hmnIs102 [col-56pro:tra-2;
unc-122pro:GFP]; hmnEx2422 [pkd-2pro:GFP;
dat-1pro:mApple; rol-6(su1006)]		 This paper CHB4543
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; hmnIs102
[col-56pro:tra-2; unc-122pro:GFP]		 This paper CHB4369
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; hmnIs105
[col-56pro:fem-3; unc-122pro:GFP]		 This paper CHB4375
hmnIs47 [grl-18pro:mApple] I; him-5(e1490) V This paper CHB3515
hmnIs47 [grl-18pro:mApple] I; mab-3(hmn289) II;
him-5(e1490) V		 This paper CHB3916
hmnIs47 [grl-18pro:mApple] I; mab-3(mu15) II;
him-5(e1490) V		 This paper CHB3716
hmnIs82 [grl-18pro:GFP] II; lep-2(hmn305) IV;
him-5(e1490) V		 This paper CHB4640
hmnIs82 [grl-18pro:GFP] II; lep-2(ok900) IV;
him-5(e1490) V		 This paper CHB3897
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V;
lep-5(ny28) X		 This paper CHB3887
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V;
jmjd-3.1(hmn304) X		 This paper CHB4186
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V;
jmjd-3.1(gk384) X		 This paper CHB4248
hmnIs82 [grl-18pro:GFP] II; nfya-1(hmn316) X This paper CHB4108
hmnIs82 [grl-18pro:GFP] II; nfya-1(hmn317) X This paper CHB4109
hmnIs82 [grl-18pro:GFP] II; nfya-1(hmn319) X This paper CHB4111
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V;
nfya-1(ok1174) X; hmnEx2535 [rab-3pro:nfya-1-cDNA;
unc-122pro:RFP]		 This paper CHB4830
nfyb-1(tm4257) II; him-5(e1490) V;
hmnIs78 [grl-18pro:GFP] X		 This paper CHB4635
nfyc-1(tm4264) II; him-5(e1490) V;
hmnIs78 [grl-18pro:GFP] X		 This paper CHB4606
hmnIs82 [grl-18pro:GFP] II; bed-3(hmn318) IV;
him-5(e1490) V		 This paper CHB4289
hmnIs82 [grl-18pro:GFP] II; bed-3(sy705) IV;
him-5(e1490) V		 This paper CHB4249
hmnIs82 [grl-18pro:GFP] II; bed-3(gk996) IV;
him-5(e1490) V		 This paper CHB4400
grl-18(hmn340 [grl-18:SL2:YFP:H2B + loxP])
him-5(e1490) V		 This paper CHB4470
hmnEx2223 [col-56pro:GFP; dat-1pro:mApple;
rol-6(su1006)]		 This paper CHB3992
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; myEx696
[pdf-1pro:RFP; unc-122pro:GFP]		 This paper CHB4284
hmnIs82 [grl-18pro:GFP] II; him-5(e1490) V; nfya-1(ok1174) X; myEx696 [pdf-1pro:RFP; unc-122pro:GFP] This paper CHB4246
him-5(e1490) V; kyIs53 [odr-10:GFP] X This paper CHB4360
him-5(e1490) V; kyIs53 [odr-10:GFP] nfya-1(ok1174) X This paper CHB4352
hmnIs47 [grl-18pro:mApple] I; grl-18(hmn341) him-5(e1490) V; hmnEx2398 [grl-18pro:sfGFP:grl-18-gDNA(352–445); rol-6(su1006)] This paper CHB4848
grl-18(hmn341) him-5(e1490) V; hmnEx2422 [pkd-2pro:GFP; dat-1pro:mApple; rol-6(su1006)] This paper CHB4567
grl-18(ok2845) him-5(e1490) V; hmnEx2422 [pkd-2pro:GFP; dat-1pro:mApple; rol-6(su1006)] This paper CHB4583
unc-119(ox819) III; hmnEx2467 [mam-5pro2:grl-18-gDNA; unc-119(+)] This paper CHB4600
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV;
mab-23(gk664) V		 This paper CHB3910
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV;
dmd-3(ok1327) V		 This paper CHB3828
hmnIs47 [grl-18pro:mApple] I; dmd-5(ok1394) II;
him-8(e1489) IV		 This paper CHB3844
hmnIs47 [grl-18pro:mApple] I; dmd-6(gk287) IV;
him-5(e1490) V		 This paper CHB3686
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV;
dmd-7(ok2276) V		 This paper CHB3768
hmnIs47 [grl-18pro:mApple] I; dmd-8(ok1294) V This paper CHB3769
hmnIs47 [grl-18pro:mApple] I; dmd-9(ok1438) IV;
him-5(e1490) V		 This paper CHB3717
hmnIs47 [grl-18pro:mApple] I; him-8(e1489) IV;
dmd-10(gk1125) V		 This paper CHB3901
Oligonucleotides
See Table S2 for oligonucleotide information. N/A
Recombinant DNA
mir-228pro:fem-3 This paper pTT03
col-56pro:GFP This paper pWF1
mir-228pro:tra-2 This paper pWF4
pkd-2pro:GFP This paper pWF8
grl-18pro:grl-18-gDNA:sfGFP This paper pWF15
grl-18pro:sfGFP:grl-18-gDNA This paper pWF16
dat-1pro:mApple This paper pWF17
col-56pro:fem-3 This paper pWF22
col-56pro:tra-2 This paper pWF23
col-56pro:nfya-1-cDNA This paper pWF53
nfya-1pro:nfya-1-cDNA This paper pWF54
SapTrap_grl-18:SL2:YFP:H2B This paper pWF107
grl-18pro:sfGFP:grl-18-gDNA(31–351) This paper pWF114
grl-18pro:sfGFP:grl-18-gDNA(352–445) This paper pWF115
pU6:grl-18-sgRNA #1 This paper pWF125
pU6:grl-18-sgRNA #2 This paper pWF126
mam-5pro2:grl-18-gDNA This paper pWF136
rab-3pro:nfya-1-cDNA This paper pWF147
Software and algorithms
CRISPOR Concordet et al., 201871 http://37.187.154.234/crispor.py
MiModD Wolfgang Maier72 Version 0.1.9
Galaxy Afgan et al., 201873 https://github.com/galaxyproject/galaxy/
Softworx Applied Precision Version 5.5
ImageJ (Fiji) Schindelin et al., 201274 http://fiji.sc/
Affinity Photo Serif Version 1.10.1
Maps Thermo Fisher Scientific Versions 3.11 and 3.17
IMOD Kremer et al., 199675 https://bio3d.colorado.edu/imod/
GraphPad Prism GraphPad Version 9
Other
DeltaVision Applied Precision N/A
Helios SEM microscope Thermo Fisher Scientific N/A