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. Author manuscript; available in PMC: 2023 Oct 16.
Published in final edited form as: J Mol Biol. 2022 Oct 13;435(1):167853. doi: 10.1016/j.jmb.2022.167853

Figure 3. Mutations of the primary and tripartite interfaces.

Figure 3.

(A) Close-up view of the polybasic region of Syt1 C2B with residues Lys322, Lys325, Lys326, Lys327 shown as sticks (same view as in the left panel of Figure 2(A)). (B) Close-up view of the primary interface with residues SNAP-25A Lys40, Asp166, Val48 (DEE mutations) and Syt1 C2B Arg281, Glu295, Tyr338, Arg398, Arg399 (quintuple mutant) shown as sticks. (C) Close-up view of the tripartite interface with residues Syt1 C2B Thr383, Gly384, Leu387, Leu394, Phe349 and syntaxin-1A Glu211 shown as sticks. For all illustrations the crystal structure (PDB ID 5W5C) was used.