Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1962 Mar;10(2):93–98. doi: 10.1128/am.10.2.93-98.1962

Production Model Press for the Preparation of Bacterial Cell Walls

T D Perrine 1, E Ribi 1, W Maki 1, B Miller 1, E Oertli 1
PMCID: PMC1057819  PMID: 14485524

Abstract

A modification of the apparatus previously described permits the preparation of cell walls in quantity. This consists of a heavy duty, double-acting hydraulic press with motor-driven pump, and a superstrength alloy steel pressure cell which is corrosion resistant. Liquid cooling of the jet is substituted for the previously used gas cooling to minimize aerosol formation and to facilitate subsequent treatment of the products. The device produces cell walls of excellent quality in good yield. The pressure cell has been used satisfactorily up to about 60,000 psi. Design details are given.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. RIBI E., PERRINE T., LIST R., BROWN W., GOODE G. Use of pressure cell to prepare cell walls from Mycobacteria. Proc Soc Exp Biol Med. 1959 Mar;100(3):647–649. doi: 10.3181/00379727-100-24730. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES