Figure 2. ArcB dimer model by AlphaFold2 multimer.

(A) The ArcB dimer model is shown; in magenta, one monomer, and in cyan, the other monomer, orientation is intended to show the position of all domains, as in Fig. 1. (B) Leucine zipper residues are indicated in both monomers in red. In the zoomed region, the cyan box indicates the residue that, when mutated, leads to the loss of regulation and, in magenta, the loss of kinase activity in vitro (Matsushika & Mizuno, 2000; Nuñez Oreza et al., 2012). Black arrows indicate the residues that mutations lead to a dominant negative phenotype in vivo. Leucine numbering is displayed on the right of the figure.