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. 2023 Sep 8;8(17):e170387. doi: 10.1172/jci.insight.170387

Figure 3. Postnatal hypertrophic growth of skeletal muscle requires SEL1L-HRD1 ERAD.

Figure 3

(A) Representative photos of hindlimb skeletal muscle tissue in mice at 4 and 12 weeks old. (B) Comparison of glycolytic (gastrocnemius, tibialis anterior, EDL) and oxidative muscle (soleus) mass of 4- and 12-week-old male mice (n = 6 per genotype/time point). (C) Representative H&E staining of tibialis anterior muscle in 4- and 12-week-old mice (n = 6 mice per genotype). (D) Representative confocal image of laminin staining of 8-week-old tibialis anterior muscle (n = 4 mice per genotype). (E) Frequency distribution of muscle cross-sectional area of 4- and 12-week-old tibialis anterior muscle with quantitation (n = 6 mice per genotype/time point, ~100–200 fibers per mouse). (F) Fiber type analysis of myosin heavy chain (MyHC) subtypes, type I (red), type IIa (blue), type IIx (unstained/black), and type IIb (green), with laminin staining the myofiber membrane (n = 5–6 mice per genotype). (G) Quantitation of fiber type percentage of tibialis anterior muscle from 8-week-old mice (n = 5–6 mice per genotype). (H) Body mass curves of male and female Ire1αfl/fl and Ire1αMLC mice (n = 4–28 per genotype/sex/time point). (I) Comparison of glycolytic (quadriceps, gastrocnemius, tibialis anterior [T.A.], EDL) and oxidative muscle (soleus [SOL]) mass of 15-week-old male mice (n = 3 mice per genotype). Data presented as mean ± SEM. NS, P > 0.05; *P < 0.05; **P < 0.01; ****P < 0.0001 determined by 2-way ANOVA with Tukey’s multiple-comparison test (B and E), 2-tailed, unpaired t test (G and I), or mixed-effects analysis (repeated-measure ANOVA) (H).