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. 2023 Oct;29(10):1471–1480. doi: 10.1261/rna.079693.123

FIGURE 3.

FIGURE 3.

The TEX15 DUF3715 domain can functionally substitute TASOR's DUF3715 domain and mediate transposon silencing. (A) Schematic representation of human TASOR variants expressed in Tasor−/− ESCs. All proteins contain an amino-terminal HA tag. (B) Representative image of western blot of mESC lysates from wild-type and Tasor−/−, with or without reconstitution as indicated (n = 2). (C,D) Representative immunofluorescence (IF) images of wild-type and Tasor−/− with or without reconstitution as indicated (n = 2). DNA was stained with DAPI. (C) mESCs stained for HA (TASOR). Scale bars indicate 10 µm. (D) mESCs stained for LINE1 ORF1p. Scale bars indicate 5 µm. (E) Data were acquired by RNA-seq from mESCs. Comparison of transposon expression between control and Tasor−/− with or without reconstitution as indicated (n = 2). Heat map shows the fold change of the 10 most up-regulated LINE1s in Tasor−/− (P < 0.01, Benjamini–Hochberg adjusted two-sided Wald test).