TABLE 3.
Comparison of the histological and laboratory characteristics of mice fed with chow or high-fat, high-sucrose diet
| Genetic background | C57BL/6J (Ldlr-/-) | C57BL/6J (Ldlr-/-) |
|---|---|---|
| Diet | Normal chow | HFHS |
| Duration | 20 wks | 20 wks |
| Number of mice | 7–9 | 9 |
| Body weight | 28.3 ± 1.2 | 52.02 ± 1.2a |
| Plasma levels (fasting) | ||
| Glucose | 226 ± 13.5 | 278.2 ± 16.0a |
| AST (pg/ml) | 454.3 ± 100.2 | 934.2 ± 75.1a |
| Cholesterol (mg/dl) | 221.1 ± 13.74 | 1462.1 ± 56.74a |
| Triglyceride (mg/dl) | 338.1 ± 13.74 | 1176.9 ± 15.14a |
| Insulin (ng/ml) | 0.56 ± 0.164 | 5.51 ± 2.16a |
| Hepatic histology, n (%) | ||
| Steatosis gradea | ||
| 0 | 7 (100) | 0 |
| 1 | 0 | 0 |
| 2 | 0 | 9 (100) |
| 3 | 0 | 0 |
| Inflammation gradea | ||
| 0 | 7 (100) | 4 (44) |
| 1 | 0 | 5 (56) |
| 2 | 0 | 0 |
| 3 | 0 | 0 |
| Fibrosis stage, n (%) | ||
| 0 | 7 (100) | 6 (67) |
| 1 | 0 | 3 (33) |
| 2 | 0 | 0 |
| 3 | 0 | 0 |
a
Notes: Data are expressed as mean ± SEM, number within parenthesis represents the percentage of mice. p-value from unpaired 2-tailed nonparametric Mann-Whitney test (p<0.05, difference between HF and HFHS). HFHS diet with added 0.15% dietary cholesterol. Statistical analysis using Student t-test.
Abbreviations: HF, high fat; HFHS, high-fat, high-sucrose.