TABLE 1.
Mutations within the grlA, grlB, gyrA, and gyrB genes in 116 clonally unrelated clinical isolates of S. aureus from eight countries
| Gene and base position(s) (change[s])a | Amino acid change | No. of strains
|
|||
|---|---|---|---|---|---|
| Ciprofloxacin susceptible | Ciprofloxacin resistant | MSSA | MRSA | ||
| grlA | |||||
| 2495 (A→C)* | None | 2 | 0 | 2 | 0 |
| 2614 (C→T) | Ser-80→Phe | 1 | 60 | 1 | 60 |
| 2651 (C→T)* | None | 3 | 0 | 3 | 0 |
| 2756 (A→G)* | None | 1 | 0 | 0 | 1 |
| 2614 (A→C); 2626 (A→T)* | Ser-80→Phe; Glu-84→Val | 0 | 4 | 0 | 4 |
| 2614 (A→C); 2751 (T→C)* | Ser-80→Phe; none | 0 | 1 | 0 | 1 |
| 2517 (G→A); 2614 (C→T)* | Ala-48→Thr; Ser-80→Phe | 0 | 4 | 1 | 3 |
| 2510 (T→G); 2516 (T→C); 2606 (T→C)* | Ile-45→Met; none; none | 2 | 0 | 2 | 0 |
| 2714 (G→A); 2798 (T→C)*; 2805 (C→T); 2825 (A→G)* | None; none; Pro-144→Ser; none | 4 | 0 | 2 | 2 |
| 2510 (T→G); 2516 (T→C)*; 2606 (T→C); 2614 (C→T)* | Ile-45→Met; none; none; Ser-80→Phe | 1 | 0 | 1 | 0 |
| 2497 (T→G); 2510 (T→G); 2516 (T→C)*; 2606 (T→C); 2614 (C→T)* | Val-41→Gly; Ile-45→Met; none; none; Ser-80→Phe | 0 | 1 | 1 | 0 |
| No mutations | 32 | 0 | 10 | 22 | |
| Total | 46 | 70 | 23 | 93 | |
| grlB | |||||
| 1644 (C→T)* | None | 1 | 0 | 1 | 0 |
| 1650 (A→T)* | Glu-422→Asp | 1 | 0 | 1 | 0 |
| 1677 (T→C)* | None | 1 | 6 | 0 | 7 |
| 1679 (A→G)* | Asp-432→Gly | 0 | 1 | 0 | 1 |
| 1735 (C→T)* | Pro-451→Ser | 0 | 1 | 0 | 1 |
| 1866 (T→C)* | None | 1 | 0 | 0 | 1 |
| 1650 (A→T); 1776 (A→G); 1866 (T→C)* | Glu-422→Asp; none; none | 1 | 0 | 1 | 0 |
| 1644 (C→T); 1650 (A→T); 1710 (A→T)*; 1770 (A→T); 1866 (T→C)* | None; Glu-422→Asp; none; none; none | 3 | 1 | 4 | 0 |
| 1644 (C→T); 1647 (T→A); 1650 (A→T)*; 1776 (A→G); 1866 (T→C)* | None; none; Glu-422→Asp; none; none | 3 | 0 | 2 | 1 |
| No mutations | 35 | 61 | 14 | 82 | |
| Total | 46 | 70 | 23 | 93 | |
| gyrA | |||||
| 2402 (C→T) | Ser-84→Leu | 0 | 43 | 1 | 42 |
| 2409 (T→C) | None | 16 | 0 | 3 | 13 |
| 2413 (G→A) | Glu-88→Lys | 1 | 0 | 1 | 0 |
| 2481 (T→C) | None | 2 | 1 | 3 | 0 |
| 2487 (A→G) | Ser-112→Arg | 1 | 0 | 1 | 0 |
| 2402 (C→T); 2409 (T→C) | Ser-84→Leu; none | 0 | 8 | 1 | 7 |
| 2402 (C→T); 2481 (T→C)* | Ser-84→Leu; none | 0 | 1 | 1 | 0 |
| 2409 (T→C); 2413 (G→A)* | None; Glu-88→Lys | 0 | 16 | 0 | 16 |
| 2402 (C→T); 2409 (T→C); 2468 (G→A)* | Ser-84→Leu; none; Gly-106→Asp | 0 | 1 | 0 | 1 |
| No mutations | 26 | 0 | 12 | 14 | |
| Total | 46 | 70 | 23 | 93 | |
| gyrB | |||||
| 1534 (G→A) | None | 2 | 0 | 2 | 0 |
| 1579 (A→G)* | None | 16 | 26 | 7 | 35 |
| 1525 (C→T); 1579 (A→G)* | None; none | 1 | 0 | 0 | 1 |
| 1474 (T→A); 1498 (C→T)* | None; none | 1 | 0 | 1 | 0 |
| No mutations | 26 | 44 | 13 | 57 | |
| Total | 46 | 70 | 23 | 93 | |
a
*, new single point mutations or combinations of single point mutations.