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. 2023 Oct 16;13(11):373. doi: 10.1007/s13205-023-03791-w

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Fig. 11 — Detection of PVA in field samples of potato (tubers) by one-step RT-RPA and RT-PCR. A One-step RT-PCR was carried out following earlier reported primers (Meena et al. 2017) and purified RNA, B One-step RT-PCR was carried out with primer pair A and purified RNA, C One-step RT-RPA was carried out with primer pair A and purified RNA, D One-step RT-RPA was carried out with primer pair A and cellular disc paper-based RNA extract with 8 M LiCl. Lane L-1Kb or 100 bp ladder, Lane 1–15 shows different samples of potato tubers, PC- known positive tuber sample of potato, HC-healthy potato tuber sample, NC-Negative (water) control