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PVA detection by RT-PCR using three newly designed primer pairs (A–C). In lanes L-1 Kb or 100 bp ladder, primer set A (PVARPAF1/R1) was expected to give a product of 185 bp; primer set B (PVARPAF1/R2) was expected to produce a product of 176 bp; primer set C (PVARPAF2/R1) was expected to produce a product of 183 bp; lane NC is a negative (water) control
