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. 2023 Oct 16;13(11):373. doi: 10.1007/s13205-023-03791-w

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© King Abdulaziz City for Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 6 — The optimization of incubation period and magnesium acetate concentrations in the RT-RPA reaction for the detection of PVA using primer pair A (PVARPAF1/R1). In A, cDNA is used as a template in a thermal cycler. In B, cDNA is used as template in the water bath/heating block. In C, RNA is used as a template, under a thermal cycler. D Using RNA as a template and a water bath/heating block. E RNA was used as a template for determining the optimum concentration of magnesium acetate, under thermal cycler. F RNA was used as a template for determining the optimum concentration of magnesium acetate, under water bath/heating block. Lane L—1 Kb or 100 bp ladder, lane HC healthy control (cDNA/RNA)