Table 2.

An analysis of the one-step reverse transcription-polymerase chain reaction (RT-PCR) and reverse transcription-recombinase polymerase amplification (RT-RPA) in potato samples (leaves and tubers) from different states of India to compare and validate the presence of potato virus A (PVA) in the samples

Field Sample No	One-step RT-PCR (Meena et al. 2017)		One-step RT-PCR with primer pair A (PVARPAF1/R1)		One-step RT-RPA with primer pair A (PVARPAF1/R1) under heating block/ water bath		One-step RT-RPA with primer pair A (PVARPAF1/R1) under heating block/ water bath
	Purified RNA		Purified RNA		Purified RNA		Simple RNA without LiCl	Simple RNA with LiCl
	Leaf	Tuber	Leaf	Tuber	Leaf	Tuber	Leaf	Tuber
1	+	+	+	+	−	+	−	+
2	+	+	+	+	+	+	+	+
3	+	+	−	+	−	+	−	+
4	+	+	+	+	+	−	+	−
5	+	+	+	+	+	+	+	+
6	+	+	+	+	+	+	+	+
7	+	+	+	−	+	−	+	−
8	+	+	+	+	−	−	−	−
9	+	+	+	+	+	+	+	+
10	+	+	+	+	+	+	+	+
11	+	+	+	+	+	+	+	+
12	+	+	+	+	+	+	+	+
13	−	+	−	+	−	+	−	+
14	+	+	+	+	+	+	+	+
15	+	+	+	+	+	+	+	+
PC	+	+	+	+	+	+	+	+
HC	−	−	−	−	−	−	−	−
NC	−	−	−	−	−	−	−	−

PC known positive leaf/tuber sample of potato, HC healthy potato leaf/tuber sample, NC negative (water) control,  + PVA positive; − PVA negative