Table 3.
A comparative study of double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), reverse transcription-polymerase chain reaction (RT-PCR), and reverse transcription-recombinase polymerase amplification (RT-RPA) methods for the detection of potato virus A (PVA) in field-collected suspected plant parts collected across agroecological zones in different states of India (including both the leaves and tubers of the same plant)
| Sample No | Location/state | Cultivar/variety | DAS-ELISA | One-step RT-PCR (Meena et al. 2017) | One-step RT-RPA with primer pair A (PVARPAF1/R1) under heating block/water bath | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Purified RNA | Purified RNA | Simple RNA without LiCl | Simple RNA with LiCl | ||||||||
| Leaves | Dormant tubers | Sprouted tubers | Leaves | Dormant tubers | Leaves | Dormant tubers | Leaves | Dormant tubers | |||
| 1 | Gujarat | Laddy Rosetta | − | − | − | − | − | − | − | − | − |
| 2 | Gujarat | Kufri Pukhraj | + | − | + | + | + | + | + | + | + |
| 3 | Gujarat | Kufri Jyoti | + | − | − | + | + | + | + | + | + |
| 4 | Gujarat | Kufri Sindhuri | − | − | − | − | − | − | − | − | − |
| 5 | Punjab | Kufri Pukhraj | − | − | − | − | − | + | − | + | − |
| 6 | Punjab | Kufri Badshah | − | − | − | + | + | + | + | + | + |
| 7 | Punjab | Laddy Rosetta | − | − | − | − | − | − | − | − | − |
| 8 | Punjab | Kufri Ashoka | + | − | − | + | + | + | + | + | + |
| 9 | Haryana | Kufri Chandramukhi | + | − | − | + | + | + | + | + | + |
| 10 | Haryana | Kufri Jyoti | + | − | + | + | + | + | + | + | + |
| 11 | Haryana | Kufri Bahar | − | − | − | − | − | − | − | − | − |
| 12 | Haryana | Kufri Jawahar | − | − | − | + | + | + | + | + | + |
| 13 | Himachal Pradesh | Kufri Jyoti | − | − | − | − | − | − | − | − | − |
| 14 | Himachal Pradesh | Kufri Himalini | − | − | − | − | − | − | − | − | − |
| 15 | Himachal Pradesh | Kufri Chandramukhi | + | − | + | + | + | + | + | + | + |
| 16 | Himachal Pradesh | Kufri Shailja | + | − | − | + | + | + | + | + | + |
| 17 | Uttar Pradesh | Kufri Pukhraj | − | − | − | + | − | + | + | + | + |
| 18 | Uttar Pradesh | Kufri Chipsona 1 | + | − | + | + | + | + | + | + | + |
| 19 | Uttar Pradesh | Kufri Chipsona 2 | − | − | − | + | − | + | + | + | + |
| 20 | Uttar Pradesh | Kufri Bahar | + | − | − | + | − | + | + | + | + |
| 21 | Bihar | Kufri Sindhuri | + | − | − | + | + | + | + | + | + |
| 22 | Bihar | Kufri Chandramukhi | − | − | − | + | - | + | + | + | + |
| 23 | Bihar | Kufri Anand | + | − | + | + | + | + | + | + | + |
| 24 | Bihar | Kufri Sindhuri | − | − | − | − | − | − | − | − | − |
| 25 | Madhya Pradesh | Kufri Pukhraj | + | − | + | + | + | + | + | + | + |
| 26 | Madhya Pradesh | Kufri Lauvkar | − | − | − | + | + | + | + | + | + |
| 27 | Madhya Pradesh | Kufri Chandramukhi | + | − | − | + | + | + | + | + | + |
| 28 | Madhya Pradesh | Kufri Surya | − | − | − | − | − | − | − | − | − |
| 29 | West Bengal | Kufri Jyoti | + | − | − | + | + | + | + | + | + |
| 30 | West Bengal | Kufri Chandramukhi | − | − | − | − | − | − | − | − | − |
| 31 | West Bengal | Kufri Pukhraj | + | − | − | + | + | + | + | + | + |
| 32 | West Bengal | Kufri Ashoka | + | − | + | + | + | + | + | + | + |
| 33 | Meghalaya | Kufri Megha | − | − | − | − | − | − | − | − | − |
| 34 | Meghalaya | Kufri Giriraj | + | − | + | + | + | + | + | + | + |
| 35 | Meghalaya | Kufri Jyoti | − | − | − | − | − | − | − | − | − |
| Total | 18 | 0 | 08 | 23 | 19 | 25 | 23 | 25 | 23 | ||
+ PVA positive; − PVA negative