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Method rankings based on validation parameters, and an overall cumulative score of each.
| Repeatability | Metabolome coverage | Metabolite recovery | Cumulative score | |
|---|---|---|---|---|
| Method 1 (original in-house) | 4 | 5 | 4 | 13 |
| Method 2 (acetonitrile addition) | 5 | 2 | 2 | 9 |
| Method 3 (two-step derivatization) | 2 | 4 | 5 | 11 |
| Method 4 (combination of Methods 2 & 3) | 3 | 3 | 3 | 9 |
| Method 5 (direct analysis) | 1 | 1 | 1 | 3 |
