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. 2023 Aug 26;17(11):1884–1894. doi: 10.1038/s41396-023-01499-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Overview of P. schiedti cell with the nucleus (N); the different arrowheads indicate different prokaryotic morphotypes; Open arrowheads point to putative Ca. Vesiculincola pelomyxae cells, while white filled arrowheads point to putative Ca. Methanoregula pelomyxae cells. B Detail of the nucleus and the bacterial symbionts located around it; white arrowheads point to putative Ca. Methanoregula pelomyxae cells while black filled arrowheads point to putative MROs. C, D Detail of the bacterial symbionts located in the cytosol (black filled arrowheads) and inside the vacuole (white arrowheads). The bacterial cells in the vacuole (C) are in the process of endospore formation; the diameter of the sporangia is considerable larger than that of the vegetative cells (D) The black filled arrowheads in C and D point to putative Ca. Syntrophus pelomyxae cells. Scale bar: A – 5 µm; B – 2 µm; C, D – 500 nm.