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. 2023 Oct 16;9:378. doi: 10.1038/s41420-023-01675-9

Fig. 9. HOXA5 promotes SPRY2 transcription by binding to the promoter of SPRY2.

Fig. 9

A SPRY2 mRNA and protein levels in HOXA5-overexpressed and HOXA5-silenced PCa cells. B SPRY2 promoter reporters were co-transfected with HOXA5 overexpressed plasmid into 293T cells. The SPRY2 promoter activity was determined by a dual luciferase assay kit. C ChIP assay showed the binding of HOXA5 to the promoter of SPRY2 in PCa cells. The decreased cell proliferation (D), cell invasion (E), phospho-ERK expression (F), and percentage of CD133+ stem cell subpopulation (G) caused by HOXA5 overexpression were partially rescued by the transfection with SPRY2-shRNA. *P < 0.05, **P < 0.01. #P < 0.05, ##P < 0.01. Results were presented as mean ± SD.