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. 2023 Oct 16;14:6515. doi: 10.1038/s41467-023-42202-9

Fig. 1. Identification of the descending SDGs that innervate C4da axon terminals.

Fig. 1

a Top, a schematic lateral view of C4da neurons in third instar larvae. Bottom, a schematic ventral view of axon terminal projections of C4da neurons in the ventral nerve cord (VNC). b Cell bodies of SDGs located in the larval brain. Nuclear-targeted GFP (green) and membrane-bound tdTomato (magenta) were driven by SDGs-spGAL4. Similar results were obtained across 3 independent samples per genotype. c Dual labeling of SDGs expressing mCD8::GFP (green) and C4da neurons visualized with ppk-CD4::tdTomato (magenta). Top panels show the signals in the larval brain and VNC. Middle panels show magnified images of the abdominal A6–7 segments in the VNC (the yellow squared area in the top panels). Bottom panels are transverse sections of the A7 segment. Similar results were obtained across 5 independent samples per genotype. d Expression of the presynaptic marker Brp::mCherry (magenta), and mCD8::GFP (green) under the control of SDGs-spGAL4. Similar results were obtained across 5 independent samples per genotype. e Left, a schematic view of Syb-GRASP signals between SDGs and C4da neurons. Middle, confocal images of Syb-GRASP signals in the larval VNC. Right, the axon terminals of SDGs are labeled by CsChrimson::mCherry. Note that each pair of images placed side-by-side shows an identical region. Similar results were obtained across 4 independent samples per genotype. See Supplementary Table 1 for full genotypes. f A schematic image of synaptic contacts from SDGs to C4da axon terminals.