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. 2023 Sep 8;17(11):1993–2002. doi: 10.1038/s41396-023-01506-w

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Fig. 2 — A The Shannon index of ARGs in WWTP effluent and river habitats (FL: free-living fraction, PA: particle-associated fraction). Those habitats which share the same letter (i.e., a or b or c) are not statistically different from each other. B Non-metric multidimensional scaling (NMDS) analysis of the resistomes of various riverine compartments (waters, biofilms, sediments, and freshwater amphipod guts) and WWTP effluents (final stress = 0.168). The selected ARGs were scaled by the square root of R². The variance ellipses using standard deviation of point scores (with confidence limits of 0.95) were highlighted in different colors for biofilm (in yellow), wastewaters (free-living in thick red; suspended particle in thick blue), river waters (free-living in light red; suspended particle in light blue), sediment (in black), and amphipod guts (in gray). The ARGs that are significantly correlated with the ordination (p ≤ 0.001) were displayed with the symbol × in red.