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. 2023 Sep 26;26(10):108056. doi: 10.1016/j.isci.2023.108056
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rat anti-Embigin G7.43.1 Invitrogen Cat# 14-5839-81, RRID: AB_2016582
Rabbit anti-Embigin Novus Cat# NBP2-24689, RRID:AB_2941092
Mouse anti-PMCA 5F10 Thermo Fisher Scientific Cat# MA3-914, RRID:AB_2061566
Mouse (IgG1) anti-Ctbp BD Cat# 612044, RRID: AB_399431
Mouse (IgG2a) anti-GluR2 Millipore Cat# MAB397, RRID: AB_2113875
Rabbit anti-Myosin7a Proteus Biosciences Cat# 25–6790, RRID: AB_10015251
Mouse anti-β-actin Proteintech Cat# 60008-1-Ig, RRID:AB_2289225
Mouse anti-β-Tubulin III Sigma-Aldrich Cat# 25–6790, RRID: AB_10015251
Alexa Fluor 647 donkey anti-rabbit Thermo Fisher Scientific Cat# A-21244, RRID:AB_2535812
Alexa Fluor 594 donkey anti-Rat Thermo Fisher Scientific Cat# A-21209, RRID: AB_2535795
Alexa Fluor 488 donkey anti-mouse Thermo Fisher Scientific Cat# A-21202, RRID:AB_141607
Alexa Fluor 568 goat anti- mouse IgG1 Thermo Fisher Scientific Cat# A21124, RRID:AB_141611
Alexa Fluor 488 goat anti- mouse IgG2a Thermo Fisher Scientific Cat# A21131, RRID:AB_2535771
Dylight 405 AffiniPure donkey anti-rabbit IgG Jackson Immuno Research Labs Cat# 711-475-152, RRID:AB_2340616
HRP-conjugated goat anti-Rat Invitrogen Cat# 31470, RRID:AB_228356
Donkey anti-mouse IRDye 800CW LI-COR Biosciences Cat# 926–32212, RRID:AB_621847
Goat anti-rat IRDye 680RD LI-COR Biosciences Cat# 926–68076, RRID:AB_10956590

Experimental models: Organisms/strains

C57BL/6N-Embtm1b(KOMP)Wtsi Mutant Mouse Resource and Research Center (MMRRC) RRID:MGI:5767650
C57BL/6NTac Mary Lyon Center at Harwell RRID:IMSR_TAC:B6
Cdh23ahl+em3H/Cdh23753A>G Mary Lyon Center at Harwell RRID:MGI:5749861

Chemicals, peptides, and recombinant proteins

PNGase F NEB Cat# P0704S
X-Gal Thermo Scientific Cat# R0404
Pro-Long Gold Antifade Mountant Invitrogen Cat# P36930
Dynabeads™ Protein G Invitrogen Cat# 10003D

Critical commercial assays

High-Capacity RNA-to-cDNA™ Kit Applied Biosystems Cat# 4387406
Emb TaqMan Assay Thermo Fisher Scientific assay ID Mm00515881_m1
Hprt TaqMan Assay Thermo Fisher Scientific assay ID Mm03024075_m1

Deposited data

Mouse Brain Atlas Allen Brain Atlas https://mouse.brain-map.org/
CDH23 Expression map Human Protein Atlas https://www.proteinatlas.org
Ensembl (release 109) Mouse reference genome GRCm39 ensembl.org https://www.ensembl.org

Software and algorithms

BiosigRZ (version 5.7.1) Tucker Davies Technology https://www.tdt.com/component/biosigrz-abr-dpoae-software/
GraphPad Prism 9.0 GraphPad by Dotmatics https://www.graphpad.com/
NDP.view2 Image viewer Hamamatsu Cat# U12388-01
ZEN Microscopy Software Zeiss https://www.zeiss.com/microscopy/en/products/software/zeiss-zen.html
Fiji ImageJ https://imagej.net/software/fiji/
NRecon Bruker Micro-CT Software https://blue-scientific.com/bruker-micro-ct/micro-ct-software/

Other

TDT RZ6 System 3 Tucker Davies Technology https://www.tdt.com/products/
NanoZoomer Slide Scanner Hamamatsu NanoZoomer 2.0 -RS, discontinued.
Confocal Microscope Zeiss Model: LSM 710
Scanning Electron Microscope JEOL JSM 6010LV
Chemiluminescent imager (Western blot) UVP ChemiDoc-It
Fluorescent imager (Western blot) LI-COR Odyssy DLx Imager
X-ray micro-CT system Bruker Skyscan 1172 scanner