| Antibodies |
|
| Rat anti-Embigin G7.43.1 |
Invitrogen |
Cat# 14-5839-81, RRID: AB_2016582
|
| Rabbit anti-Embigin |
Novus |
Cat# NBP2-24689, RRID:AB_2941092
|
| Mouse anti-PMCA 5F10 |
Thermo Fisher Scientific |
Cat# MA3-914, RRID:AB_2061566
|
| Mouse (IgG1) anti-Ctbp |
BD |
Cat# 612044, RRID: AB_399431
|
| Mouse (IgG2a) anti-GluR2 |
Millipore |
Cat# MAB397, RRID: AB_2113875
|
| Rabbit anti-Myosin7a |
Proteus Biosciences |
Cat# 25–6790, RRID: AB_10015251
|
| Mouse anti-β-actin |
Proteintech |
Cat# 60008-1-Ig, RRID:AB_2289225
|
| Mouse anti-β-Tubulin III |
Sigma-Aldrich |
Cat# 25–6790, RRID: AB_10015251
|
| Alexa Fluor 647 donkey anti-rabbit |
Thermo Fisher Scientific |
Cat# A-21244, RRID:AB_2535812
|
| Alexa Fluor 594 donkey anti-Rat |
Thermo Fisher Scientific |
Cat# A-21209, RRID: AB_2535795
|
| Alexa Fluor 488 donkey anti-mouse |
Thermo Fisher Scientific |
Cat# A-21202, RRID:AB_141607
|
| Alexa Fluor 568 goat anti- mouse IgG1 |
Thermo Fisher Scientific |
Cat# A21124, RRID:AB_141611
|
| Alexa Fluor 488 goat anti- mouse IgG2a |
Thermo Fisher Scientific |
Cat# A21131, RRID:AB_2535771
|
| Dylight 405 AffiniPure donkey anti-rabbit IgG |
Jackson Immuno Research Labs |
Cat# 711-475-152, RRID:AB_2340616
|
| HRP-conjugated goat anti-Rat |
Invitrogen |
Cat# 31470, RRID:AB_228356
|
| Donkey anti-mouse IRDye 800CW |
LI-COR Biosciences |
Cat# 926–32212, RRID:AB_621847
|
| Goat anti-rat IRDye 680RD |
LI-COR Biosciences |
Cat# 926–68076, RRID:AB_10956590
|
|
| Experimental models: Organisms/strains |
|
| C57BL/6N-Embtm1b(KOMP)Wtsi
|
Mutant Mouse Resource and Research Center (MMRRC) |
RRID:MGI:5767650 |
| C57BL/6NTac |
Mary Lyon Center at Harwell |
RRID:IMSR_TAC:B6 |
|
Cdh23ahl+em3H/Cdh23753A>G
|
Mary Lyon Center at Harwell |
RRID:MGI:5749861 |
|
| Chemicals, peptides, and recombinant proteins |
|
| PNGase F |
NEB |
Cat# P0704S |
| X-Gal |
Thermo Scientific |
Cat# R0404 |
| Pro-Long Gold Antifade Mountant |
Invitrogen |
Cat# P36930
|
| Dynabeads™ Protein G |
Invitrogen |
Cat# 10003D |
|
| Critical commercial assays |
|
| High-Capacity RNA-to-cDNA™ Kit |
Applied Biosystems |
Cat# 4387406 |
|
Emb TaqMan Assay |
Thermo Fisher Scientific |
assay ID Mm00515881_m1 |
|
Hprt TaqMan Assay |
Thermo Fisher Scientific |
assay ID Mm03024075_m1 |
|
| Deposited data |
|
| Mouse Brain Atlas |
Allen Brain Atlas |
https://mouse.brain-map.org/ |
| CDH23 Expression map |
Human Protein Atlas |
https://www.proteinatlas.org |
| Ensembl (release 109) Mouse reference genome GRCm39 |
ensembl.org |
https://www.ensembl.org |
|
| Software and algorithms |
|
| BiosigRZ (version 5.7.1) |
Tucker Davies Technology |
https://www.tdt.com/component/biosigrz-abr-dpoae-software/ |
| GraphPad Prism 9.0 |
GraphPad by Dotmatics |
https://www.graphpad.com/ |
| NDP.view2 Image viewer |
Hamamatsu |
Cat# U12388-01 |
| ZEN Microscopy Software |
Zeiss |
https://www.zeiss.com/microscopy/en/products/software/zeiss-zen.html |
| Fiji |
ImageJ |
https://imagej.net/software/fiji/ |
| NRecon |
Bruker Micro-CT Software |
https://blue-scientific.com/bruker-micro-ct/micro-ct-software/ |
|
| Other |
|
| TDT RZ6 System 3 |
Tucker Davies Technology |
https://www.tdt.com/products/ |
| NanoZoomer Slide Scanner |
Hamamatsu |
NanoZoomer 2.0 -RS, discontinued. |
| Confocal Microscope |
Zeiss |
Model: LSM 710 |
| Scanning Electron Microscope |
JEOL |
JSM 6010LV |
| Chemiluminescent imager (Western blot) |
UVP |
ChemiDoc-It |
| Fluorescent imager (Western blot) |
LI-COR |
Odyssy DLx Imager |
| X-ray micro-CT system |
Bruker |
Skyscan 1172 scanner |