Table 2.

Summary of the gas fermentation data for the four strains used in the first and second chemostat bioreactor experiments

First Fermentation Experiment – Period 2 (H2 and CO2)a
	ΔHb	ZZc	MMd
Biomass (gCDW)	0.03 ± 0.01	0.03 ± 0.00	0.04 ± 0.01
H2 Uptake Rates (mmol/gCDW/h)	234.36 ± 40.76	147.24 ± 23.88	136.12 ± 12.00
CO2 Uptake Rates (mmol/gCDW/h)	58.18 ± 11.49	34.36 ± 6.98	33.80 ± 3.78
CH4 Production Rates (mmol/gCDW/h)	52.50 ± 8.53	32.34 ± 3.92	28.83 ± 4.93

Second Fermentation Experiment (H2 and CO2 or Formate)e
	ΔHHf	ΔHFg	ZZFh
Biomass (gCDW)	0.04 ± 0.01	0.04 ± 0.01	0.04 ± 0.02
H2 Uptake Rates (mmol/gCDW/h)	127.31 ± 28.18	N/A	N/A
H2 Production Rates (mmol/gCDW/h)	N/A	0.84 ± 0.34	0.55 ± 0.28
CO2 Uptake Rates (mmol/gCDW/h)	29.16 ± 3.91	N/A	N/A
CO2 Production Rates (mmol/gCDW/h)	N/A	31.87 ± 11.12	34.29 ± 9.77
CH4 Production Rates (mmol/gCDW/h)	30.53 ± 3.97	9.69 ± 3.62	10.55 ± 3.18

The standard deviation (±) was calculated based on the entire population.

^aValues can be found in Data S3 and represent mean values and standard deviations calculated from values without gross measurement errors (STAR Methods).

^bΔH, M. thermautotrophicus ΔH.

^cZZ, M. thermautotrophicus Z-245.

^dMM, M. marburgensis Marburg.

^eValues can be found in Data S6 and represent mean values and standard deviations calculated from values without gross measurement errors (STAR Methods).

^fΔH_H, M. thermautotrophicus ΔH pMVS1111A:P_hmtB-fdh_Z-245 grown on H₂ and CO₂.

^gΔH_F, M. thermautotrophicus ΔH pMVS1111A:P_hmtB-fdh_Z-245 grown on Na-formate.

^hZZ_F, M. thermautotrophicus Z-245 grown on Na-formate.