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. 2023 Jul 25;72(11):2112–2122. doi: 10.1136/gutjnl-2023-330291

Figure 3.

Figure 3

Roseburia intestinalis cultured medium (R.i CM) exhibits tumour-suppressive effects in vitro. (A) R.i CM (5%) reduced the colonic cell viability, except NCM460. Escherichia coli conditioned medium (E. coli CM) and yeast casitone fatty acids broth (YCFA) were used as control. (B) R.i CM (5%) suppressed the colony formation of colorectal cancer (CRC) cells. (C) R.i CM reduced CRC cell Ki-67 expression levels determined by immunocytochemistry. (D) R.i CM increased CRC cell apoptosis. (E) R.i CM induced CRC cell apoptosis of CRC cells by cleaved caspase-3, cleaved caspase-7, cleaved caspase-9 and cleaved poly (ADP-ribose) polymerase (PARP) expression. (F) R.i CM inhibited CRC cell cycle G1/S transition. (G) R.i CM induced G1 arrest by Cdk6 downregulation and p27 Kip1 upregulation. (H) R.i CM inhibited the growth of the organoid derived from a patient with CRC.