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Steady-state ATPase activity and actin gliding velocity of human myosin-7a-5IQ S1 constructs
| Parameter | Long N-terminal | Short N-terminal | GFP-Long N-terminal |
|---|---|---|---|
| vmax | 0.83 ± 0.14 s−1 | 0.13 ± 0.06 s−1 | 0.1 ± 0.07 s−1 |
| Km | 27 ± 5 μM | 8 ± 1.1 μM | 8 ± 5.8 μM |
| Actin gliding velocity | 5.4 ± 0.9 nm/s | 3 ± 0.9 nm/s | 3.1 ± 0.5 nm/s |
