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. 2023 Oct 13;11:1579. Originally published 2022 Dec 23. [Version 3] doi: 10.12688/f1000research.126839.3

Figure 5. Refined Coordinates for CDS 2 of Rheb in D. yakuba.

Figure 5.

(A) The tblastn alignment for the D. melanogaster CDS 2_9850_2 (query) against the D. yakuba scaffold CM000160.2 (chr3R) placed the start of the CDS at 17,358,844 in frame +1. (B) Examination of the other lines of evidence (e.g., RNA-Seq read coverage, splice junction predictions, gene predictions, protein alignments) using the Genome Browser placed the start of the coding exon at 17,358,842. Since there are two nucleotides (GC; blue lines) prior to the first complete codon (AAA) that codes for first amino acid in the tblastn alignment (K), CDS 2_9850_2 in D. yakuba has a phase 2 splice acceptor site relative to reading frame +1.