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. 2023 Oct 13;11:1579. Originally published 2022 Dec 23. [Version 3] doi: 10.12688/f1000research.126839.3

Figure 9. The Gene Model Checker checklist indicated the presence of in-frame stop codons within coding exons 2, 3, 4, 5, 7, and 8 of the proposed gene model for chico-PB in D. eugracilis.

Figure 9.

The checklist also reports the use of a non-canonical GG splice donor site for CDS 1, and that the total length of the coding region (2,807 nt) is not divisible by three. To address these errors, the annotator should verify the annotation for the item associated with the first error in the checklist (i.e., the end coordinate for the first coding exon and its corresponding splice donor site). In this example, the end of the first coding exon should be changed from 4,678,301 to 4,678,302 based on the available evidence on the GEP UCSC Genome Browser. This change will resolve the remaining failures in the checklist.