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Observed break points in avidin fluorescence emission signals upon addition of biotin. Uncertainties are estimated using the standard deviation.
| [biotin]:[AV] at saturation λem (nm) | ||||
|---|---|---|---|---|
| λex (nm) | 335 nm | 350 nm | % diff. | N |
| 280 | 3.81 ± 0.10 | 4.00 | 5 ± 3 | 29 |
| 290 | 3.74 ± 0.18 | 4.00 | 7 ± 5 | 5 |
| [biotin]:[AV] at saturation λem (nm) | ||||
| λex (nm) | 325 nm | 350 nm | % diff. | N |
| 280 | 3.65 ± 0.08 | 4.00 | 9 ± 2 | 5 |
