Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Observed break points in various avidin fluorescence parameters upon titration with biotin aliquots (λex = 280 nm). Parameters taken directly from fits to emission fluorescence spectra and plotted as a function of ligand to protein concentration ratio. Uncertainties are estimated using the standard deviation (N = 7).
| λmax | Area | FWHM | Height | |
|---|---|---|---|---|
| [biotin]:[AV] | 4.6 | 3.8 | 4.4 | 3.3 |
| Std. dev. | 0.9 | 0.3 | 0.6 | 0.2 |
