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. 2023 Oct 3;14:1271686. doi: 10.3389/fimmu.2023.1271686

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Copyright © 2023 Hioe, Liu, Banin, Heindel, Klingler, Rao, Luo, Jiang, Pandey, Ordonez, Barnette, Totrov, Zhu, Nádas, Zolla-Pazner, Upadhyay, Shen, Kong and Hessell

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutralization activities elicited in the four rabbit groups. Neutralization of HIV-I pseudoviruses from different tiers and clades was tested in a standard assay using the TZM.bl target cells. Serum samples collected two weeks after the last immunization (week 14) were evaluated for neutralization activity. Virus pseudotyped with MLV was used as negative control, and control mAbs were also included for comparison. Bold values denote ID50 titers measurable above cut-offs (above MLV control <30 or >3-fold higher than MLV control of >30).