Table II. Summary of sequencing and sequence processing results.
| Sample | Status | Library Type | Clone Count | IgA VDJ Count | IgG VDJ Count | IgM VDJ Count |
|---|---|---|---|---|---|---|
| HD07M | HD CD27+ | Bulk | 2,620 | 1,167 | 362 | 1,676 |
| HD09M | HD CD27+ | Bulk | 2,777 | 721 | 384 | 1,900 |
| HD10M | HD CD27+ | Bulk | 3,536 | 353 | 428 | 2,894 |
| HD13M | HD CD27+ | Bulk | 3,663 | 1,036 | 945 | 2,164 |
| HD24M | HD CD27+ | Bulk | 4,576 | 3,193 | 2,440 | 705 |
| HD263M | HD CD27+ | Bulk | 10,070 | 6,569 | 3,784 | 2,223 |
| HD38M | HD CD27+ | Bulk | 3,365 | 1,152 | 966 | 1,945 |
| 141415 | ASC | 10×* | 99 | 28 | 113 | 6 |
| Donor 155 | ASC | Bulk | 5,283 | 34,365 | 87,425 | 117,910 |
| Donor 157 | ASC | Bulk | 53 | 106 | 0 | 0 |
| Donor 162 | ASC | Bulk | 2,559 | 70,562 | 92,989 | 60,018 |
| MUSK_1 | ASC | 10×a | 103 | 86 | 17 | 9 |
| MUSK_2 | ASC | 10×a | 162 | 152 | 22 | 14 |
| MUSK_3 | ASC | 10×a | 70 | 47 | 25 | 2 |
| DM1 | DM | Bulk | 1,004 | 407 | 1,289 | 35 |
| DM2 | DM | Bulk | 1,461 | 934 | 1,439 | 67 |
| DM3 | DM | Bulk | 446 | 167 | 506 | 55 |
| PM1 | PM | Bulk | 420 | 1 | 1,172 | 90 |
| PM2 | PM | Bulk | 1,076 | 702 | 991 | 59 |
| PM3 | PM | Bulk | 1,800 | 1,256 | 977 | 36 |
| IBM1 | IBM | Bulk | 654 | 716 | 502 | 144 |
| IBM2 | IBM | Bulk | 1,528 | 897 | 1,166 | 100 |
| IBM3 | IBM | Bulk | 416 | 112 | 823 | 23 |
| IBM4 | IBM | Bulk | 63 | 53 | 147 | 32 |
Counts refers to the number of unique, error-corrected sequences that passed all quality control and filtering steps. The DM, PM, and IBM samples were sequenced as part of this study. The sequences derived from samples labeled HD (healthy donor), MuSK (a myasthenia gravis disease subset), and donor were from previously reported studies (see Materials and Methods and Refs. 18 and 19 [HD], Ref. 17 [MuSK], and Ref. 20 [donor]). Sample labeled 141415 was from an unpublished study (see Materials and Methods for details).
a
For 10x Genomics–derived libraries, VDJ counts correspond directly to the absolute number of B cells.