FIGURE 2.

Coccidioides lung exposure alters blood levels of several leukocyte populations at 6–7 mo of age.

Shown is the cell frequency (millions/ml) of blood leukocyte populations in mice of each cohort: PBS (n = 14 mice), SILV (C. posadasii treated; n = 9), or RS (C. immitis treated; n = 8). (A–E) Boxplots show frequencies of (A) neutrophils, (B) total T cells, (C) total B cells, (D) nonleukemic, normal B cells (CD5⁻/B220^high), and (E) cells with a preleukemic/leukemic phenotype (CD5⁺/B220^low). Note that due to suboptimal monocyte staining in most blood samples taken at this age, insufficient data were available for statistical comparisons of monocyte numbers between cohorts. Nonetheless for two mice of each cohort, satisfactorily stained/gated for monocytes (as in Fig. 1C), the following frequencies were noted (mean ± SD in millions/ml): PBS, 0.24 ± 0.27; SILV, 1.38 ± 1.19; and RS, 0.43 ± 0.04. (F) Percent of total CD19⁺ cells with normal B cell phenotype and (G) percent of total CD19⁺ cells with leukemic phenotype. Differences were determined as statistically significant using a t test, for data with a normal distribution, or a Mann–Whitney rank-sum test for nonparametric data.