FIGURE 11.

Y188‐stained axonal blebs were derived from excitatory or inhibitory cortical neurons. Confirmation in transgenic mice 10 hr after lFPI. (A)–(C): An axonal bleb connecting its parent cell body of a YFP‐labeled pyramidal neuron (green). The axonal bleb was intensively stained with Y188 (red), showing a perfect colocalization (arrows ) of both fluorophores (orange to yellow; arrows). (D)–(F) A tdTomato‐labeled PV interneuron (red) was shown to be the parent cell body of a Y188‐stained axonal bleb (green). Although tdTomato could not perfectly label the axonal bleb, colocalization was confirmed in the parent cell body (arrows). Note the running direction of this inhibitory axon towards the pial surface of the brain, opposite to that from pyramidal neurons. Y188‐stained axonal blebs negative to the transgenic fluorescent proteins were also prominent (double arrows). Scale bars: 20 μm in (A)–(F).