Table 3.
Biocontrol efficacies of Bacillus B.PNR1 and B.PNR2 against Fusarium wilt disease in tomato
| Treatment | Approach A | Approach B | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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| Culture filtrate | Cell suspension | Culture filtrate | Cell suspension | |||||||||
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| DSSa (1–5) | DSI (%) | DC (%) | DSSa (1–5) | DSI (%) | DC (%) | DSSa (1–5) | DSI (%) | DC (%) | DSSa (1–5) | DSI (%) | DC (%) | |
| Control A | 1.0 ± 0.0 c | 0 | - | 1.0 ± 0.0 c | 0 | - | 1.0 ± 0.0 c | 0 | - | 1.0 ± 0.0 c | 0 | - |
| Control B | 5.0 ± 0.0 a | 100 | 0 | 5.0 ± 0.0 a | 100 | 0 | 5.0 ± 0.0 a | 100 | 0 | 5.0 ± 0.0 a | 100 | 0 |
| B.PNR1 | 2.4 ± 0.4 b | 48 | 52 | 2.6 ± 0.3 b | 52 | 48 | 2.6 ± 0.3 b | 52 | 48 | 5.0 ± 0.0 a | 100 | 0 |
| B.PNR2 | 2.4 ± 0.3 b | 48 | 52 | 2.4 ± 0.3 b | 48 | 52 | 4.0 ± 0.5 a | 80 | 20 | 4.8 ± 0.2 a | 96 | 4 |
Approach A: apply Bacillus 48 h before inoculating Fol, and then continuously apply Bacillus every week for 4 weeks. Approach B: apply Bacillus 48 h after inoculating Fol, and then continuously apply Bacillus every week for 4 weeks.
Control A: mock inoculation without Bacillus application; Control B: Fol TFPK 401 inoculation without Bacillus application.
DSS, disease severity score; DSI, disease index; DC, disease control efficacy.
a
Means ± standard deviation followed by different letters are significantly different according to Tukey’s range test (P < 0.05).