Table 2.
List of race-specific primers used in this study
Marker | Primer name | Sequences (5′ → 3′) | Length (bp) | Annealing conditions | Reference |
---|---|---|---|---|---|
Xcc specific | Xcc-53-F | CGGTGCCAGCGACTCGCCACG | 930 | 70°C, 20 s, 25 cycles | Rubel et al. (2019a) |
Xcc-53-R | TCCACGGCGGCGGGCCGATCTG | ||||
Xcc Race 1 specific | Xcc-47R1-F | CCTCCTGAGTCATGGCAATGGC | 1,089 | 65°C, 40 s, 20 cycles | Rubel et al. (2019b) |
Xcc-47R1-R | TAGCAGGGGAGTGCTGCTTGC | ||||
Xcc Race 2 specific | Xcc-R2-89.2-F | CGCCTTGATGTAGCCCAGTA | 929 | 58°C, 40 s, 18 cycles | Rubel et al. (2019b) |
Xcc-R2-89.2-R | GGTGGCTTGGACAACGTTC | ||||
Xcc Race 3 specific | XccR3-49-F | AAAGAGCCAATGAAGGGCGAACA | 867 | 63°C, 40 s, 25 cycles | Afrin et al. (2018) |
XccR3-49-R | TATGTCAGGCGCATAATCCGCAAT | ||||
Xcc Race 4 specific | Xcc2-46R4-F | GCGTAGCGAAAACTGGTAGTTC | 578 | 66°C, 40 s, 20 cycles | Rubel et al. (2017) |
Xcc2-46R4-R | GCACAGGCGCACCAGCATATGGC | ||||
Xcc Race 5 specific | XccR5-89.2-F | GCGAGCGATTTCACCTTCAT | 1,515 | 63°C, 40 s, 22 cycles | Afrin et al. (2019) |
XccR5-89.2-R | AATTCGGACAACAGCGCAAT | ||||
Xcc Race 6 specific | XccR6-60-F | TGTAGAATCTTTAGCTGGCGC | 693 | 63°C, 40 s, 22 cycles | Afrin et al. (2020) |
XccR6-60-R | TTCAATGCATTCCCGACACG |