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. 2023 Oct 2;2023:10.17912/micropub.biology.000996. doi: 10.17912/micropub.biology.000996

Plasmid

Reference

Notes

How to obtain plasmid

pMLS257

Schwartz et al., 2016

SapTrap destination vector for building repair template only vectors; no sgRNA

Addgene

pJW1816

Ashley et al., 2021

30aa linker::mScarlet^SEC (Lox511I)^3xMyc for SapTrap.

Addgene

pJW1877

This study

nhr-23 ::30 aa linker::mScarlet^SEC+Lox511I^3xMyc repair template. Assembled by SapTrap using pMLS257 backbone and pJW1816

Prof. Jordan Ward

pJW1856

This study

nhr-23 3' end sgRNA(F+E) vector

Prof. Jordan Ward

pJW1838

Ashley et al., 2021

SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR

Prof. Jordan Ward

pJW1776

Ragle et al., 2020

nhr-23 5' homology arm for Sap Trap (Sap sites in arm are mutated)

Prof. Jordan Ward

pJW1781

Ragle et al., 2020

nhr-23 3' homology arm for Sap Trap

Prof. Jordan Ward