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. Author manuscript; available in PMC: 2024 May 4.
Published in final edited form as: Cell Stem Cell. 2023 May 4;30(5):665–676.e4. doi: 10.1016/j.stem.2023.04.007

Figure 1. The Igfbp2-CreER strain labels zone 2 hepatocytes in a nutrient-dependent manner.

Figure 1.

A. Schema of the homology directed repair (HDR) construct used to generate the Igfbp2-CreER strain.

B. Schema of three different tamoxifen administration approaches.

C. Representative images of male Igfbp2-CreER; tdTomato het mouse livers two weeks after tamoxifen given at 100mg/kg intraperitoneally using different approaches. In magnified images, the green dashed circles represent CVs (marked by GS), and the white dashed circles represent PVs. Scale bar = 200 μm for cropped images and 100 μm for magnified images.

D. The percent area labeled in Igfbp2-CreER mice given evening tamoxifen.

E. The quantification of the Tomato+ areas of the three tamoxifen approaches. The right panel combines the data points from the two sexes shown in the left panel. The data points of evening and fasting tamoxifen are the same as in the 2-week timepoint in Figure 2C and S4D (n = 23, 24 and 5 mice for evening, fasted, and fed groups respectively).

All data in this figure are presented as mean ± SEM. Significance is displayed as p < 0.05 (*), p < 0.01 (**), p < 0.001(***), and p < 0.0001 (****).