Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-GS | Abcam | Cat#49873; RRID: AB_880241 |
| Rabbit polyclonal anti-FAH | Yecuris | Cat#20–0034 |
| Goat polyclonal anti-E-Cadherin | Bio-Techne | Cat#AF748; RRID: AB_355568 |
| Rabbit monoclonal anti-IGFBP2 | Abcam | Cat#188200 |
| Rabbit polyclonal anti-RFP | Rockland | Cat#600–401-379; RRID: AB_2209751 |
| Mouse monoclonal anti-RFP | Life technologies | Cat#MA5–15257; RRID: AB_10999796 |
| Goat anti Rabbit IgG, Alexa Fluor 488 conjugated | Life technologies | Cat#A-11008; RRID: AB_143165 |
| Donkey anti Goat IgG, Alexa Fluor 488 conjugated | Life technologies | Cat#A-11055; RRID: AB_2534102 |
| Donkey anti Rabbit IgG, Alexa Fluor 594 conjugated | Life technologies | Cat#A-21207; RRID: AB_141637 |
| Goat anti Mouse IgG1, Alexa Fluor 488 conjugated | Life technologies | Cat#A-21121; RRID: AB_2535764 |
| Goat anti Rabbit IgG, Alexa Fluor 647 conjugated | Life technologies | Cat#A-21244; RRID: AB_2535812 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat#T5648 |
| CuRx Nitisinone (NTBC) | Yecuris | Cat#20–0027 |
| 5-ethynyl-2’-deoxyuridine (EdU) | Carbosynth | Cat#NE08701 |
| Carbon tetrachloride (CCl4) | Sigma Aldrich | Cat#289116 |
| Acetaminophen | Sigma Aldrich | Cat#A7085 |
| 4% Paraformaldehyde Solution in PBS | Alfa Aesar | Cat#J19943K2 |
| Hoechst 33342 | Life technologies | Cat#H3570 |
| Citra Plus Antigen Retrieval | Fisher | Cat#NC9755543 |
| Gey’s Balanced Salt Solution | Sigma Aldrich | Cat#G9779 |
| CHAPS | Sigma Aldrich | Cat#C5070 |
| RNase inhibitor | Roche Diagnostics | Cat#3335399001 |
| Hoechst 33342 | Life technologies | Cat#H3570 |
| Critical commercial assays | ||
| Click-iT EdU Alexa Fluor 488 Imaging Kit | Life Technologies | Cat#C10337 |
| Liver Perfusion Medium | Gibco | Cat#17701038 |
| Liver Digest Medium | Gibco | Cat#17703034 |
| Hepatocyte Wash Medium | Gibco | Cat#17704024 |
| Chromium Single Cell 3’ Reagents Kit v3.1 | 10x Genomics | Cat#PN-1000269 |
| Chromium Next GEM Chip G Single Cell Kit | 10x Genomics | Cat#PN-1000127 |
| Dual Index Kit TT Set A | 10x Genomics | Cat#PN-1000215 |
| Qubit dsDNA BR Assay Kit | Invitrogen | Cat #Q32850 |
| Deposited data | ||
| Raw and analyzed snRNA-seq data | This paper | GEO: GSE228862 |
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratories | JAX: 000664 |
| Mouse: Rag1 KO; Fah KO; Il2rg KO on C57BI/6 | Yecuris | Cat #10–0001 |
| Mouse: Igfbp2-CreER | This paper | N/A |
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and algorithms | ||
| Cell Ranger software | 10x Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger |
| Scanpy package | Wolf et al., 2018 | https://github.com/theislab/Scanpy |
| GSEApy package | Fang et al., 2023 | https://github.com/zqfang/GSEApy |
| Other | ||