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. 2023 Sep 12;9(10):2025–2035. doi: 10.1021/acsinfecdis.3c00324

Figure 3.

Figure 3

Novel S-glycosides inhibit glycan biosynthesis and decrease fitness in Helicobacter pylori. (A) Western blot analysis (left) reveals diminished glycoprotein biosynthesis in H. pylori upon treatment with BacSBn 4, DATBn 5, and FucSBn 6. The short side of the triangle represents the 1 mM S-glycoside treatment, and the tall part represents the 2 mM S-glycoside treatment. Flow cytometry analysis (right) reveals increased binding of ConA lectin in H. pylori treated with S-glycosides, consistent with perturbed cell surface glycan architecture. By contrast, pretreatment of ConA with 400 mM mannose (carbo-block) prior to probing untreated H. pylori led to decreased binding. (B) Measurement of growth, viability, motility, and biofilm demonstrates a range of fitness defects in H. pylori treated with S-glycosides, with FucSBn leading to the most marked effects relative to untreated (−) controls. The data shown are representative of replicate experiments (n = 3).