Table 1. Monophosphate Metabolite to Prodrug Concentration ratios (TFV:TAF and SOF-MP:SOF) and Triphosphate Metabolite to Monophosphate Metabolite Concentration Ratios (TFV-DP:TFV and SOF-TP:SOF-MP) in Different Cell Lines after a 6-Hour Incubationa.
| Huh-7 | A549 | Vero E6 | Caco-2 | Calu-3 | Mean ± SD | |
|---|---|---|---|---|---|---|
| TFV:TAF | 45 ± 16 | 3.4 ± 1.4 | NAb | 58 ± 50 | NAb | 36 ± 29 |
| SOF-MP:SOF | 6.6 ± 4.0* | 1.9 ± 1.2 | NAb | 1.9 ± 1.2 | NAb | 3.5 ± 2.7 |
| TFV-DP:TFV | 1.8 ± 0.44 | 2.0 ± 0.2 | 1.5 ± 1.1 | 1.1 ± 0.1 | 1.3 ± 0.3 | 1.6 ± 0.3 |
| SOF-TP:SOF-MP | 0.18 ± 0.11** | NAb | 0.42 ± 0.19 | 0.36 ± 0.21** | 0.11 ± 0.03** | 0.27 ± 0.15** |
a
* P < 0.05, ** P < 0.01, n = 4 (TFV:TAF vs. SOF-MP:SOF, TFV-DP:TFV vs. SOF-TP:SOF-MP).
b
Monophosphate metabolite to prodrug concentration ratios were not determined because the analyte concentrations were below the low limits of detection after 6 h of incubation.