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. 2023 Oct 17;16:203. doi: 10.1186/s13048-023-01266-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Construction of gene co-expression networks associated with POI through WGCNA. A Determination of the soft-thresholding power (β). The left panel represents the analysis of the scale-free fit index for different soft-thresholding powers, and the right panel represent the analysis of the mean connectivity for different soft-thresholding powers. B Histogram of connectivity distribution when β = 7 and determining the scale-free topology when β = 7. C The cluster dendrogram and hierarchical clustering heatmap of module eigengenes based on the dissimilarity of TOM. Each branch represents one gene and each color represents one co-expressed module. D The heatmap plot represents interactive relationship of different module genes. E The heatmap of correlation between genes in different modules and the clinical traits. F Scatter plot of GS and MM for group in brown module. WGCNA: weighted gene co-expression network analysis; TOM: topological overlap measure; GS: gene significance; MM: module membership; FSH: follicle-stimulating hormone; AMH: anti-Mullerian hormone; LH: luteinizing hormone; E₂: estradiol; PR: progesterone; AFC: antral follicle counts; BMI: body mass index