Fig. 8.

The expression of p21 was regulated by UBE2C in human granulosa cells. A qRT-PCR showed the mRNA expression of p21 in hGCs was upregulated in bPOI patients (n = 12 in each group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Mann–Whitney U-test). B The expression of p21, caspase-3, cleaved caspase-3 and Bax after knockdown of UBE2C were measured by Western blotting. C The expression of UBE2C and p21 after overexpression of UBE2C were measured by Western blotting. Relative expression of p21 and Bax were normalized to that of the β-actin and relative expression of cleaved caspase-3 were normalized to that of caspase-3. The expression of proteins was presented as the means ± SEM from three repeated experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-tailed Student’s t-test). UBE2C: ubiquitin conjugating; SEM: standard error of mean