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. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: Nat Neurosci. 2023 Jun 5;26(7):1185–1195. doi: 10.1038/s41593-023-01353-0

Extended Data Fig. 3. Multiplex in situ hybridization (ISH) shows transcript localization for PeMP and non-PeMP transcripts.

Extended Data Fig. 3.

Maximum intensity projections of a 2 μm Z-stack image from ISH on p28 C57Bl/6 cortex, hippocampus, and corpus callosum tissue, for simultaneous PeMP-enriched vs. non-PeMP-enriched transcript detection in tandem with IBA1 immunofluorescence. Probes for PeMP-enriched transcripts shown in magenta and probes from non-PeMP-enriched transcripts shown in cyan. White arrows depict non-microglial signal emanating from somal-enriched probes, Kdm6b (a) and Ubash3b (b), while white arrowheads depict non-microglial signal emanating from PeMP-enriched probe, Plxnb1 (b). Images were also taken from negative control probes (d) to assess background signal levels. Quantifications are shown in Fig 2k–l. Representative images shown from n=5 mice and at least 4 z-stacks per brain region per mouse.