Extended Data Table 1.
Cryo-EM data collection, refinement, and validation statistics.
| Propionate-bound | |
|---|---|
| OR51E2-Gs | |
| EMDB: Full map | EMD-28896 |
| EMDB: 7TM map | EMD-28900 |
| RCSB PDB: Model | 8F76 |
| Data collection | |
| Microscope | Thermo Scientific Krios G3i |
| Detector | Gatan K3 with Gatan |
| BioQuantum Energy filter | |
| Voltage (kV) | 300 |
| Magnification | 105,000 |
| Defocus range (μm) | −1.0 to −2.1 |
| Pixel size, physical (Å) | 0.81 |
| Total exposure (e−/Å2) | 50 |
| Frame exposure (e−/Å2/frame) | 0.833 |
| Images, number of | 16,113 |
| Frames/image, number of | 60 |
| Initial particles, number of | 7,875,501 |
| Final particles, number of | 204,438 |
| Symmetry imposed | C1 |
| Map sharpening, B factor (Å2) | |
| Full map | −140.2 |
| 7TM map | −162.8 |
| Map resolution, masked (Å) | |
| Full map | 3.1 |
| 7TM map | 3.2 |
| FSC threshold | 0.143 |
| Refinement | |
| Initial model used (AlphaFold code) | Q9H255 |
| Model resolution (Å) | 3.2 |
| FSC threshold | 0.5 |
| Model composition | |
| Chains | 6 |
| Non-hydrogen atoms | 8,176 |
| Protein residues | 1,038 |
| Ligands | 1 |
| B factors (Å2) | |
| Protein | 37.96 |
| Ligand | 38.56 |
| R.m.s. deviations | |
| Bond length (Å) | 0.005 |
| Bond angles (°) | 0.873 |
| Validation | |
| MolProbity score | 1.51 |
| Clash score | 5.64 |
| EMRinger score | 3.50 |
| Rotamer outliers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 96.76 |
| Allowed (%) | 3.24 |
| Disallowed (%) | 0.00 |