TABLE 1.

Classification based on pbp1a sequence differences and properties of S. pneumoniae strains

Group	Strain	Serotype	MIC (μg/ml)a		Substitution at STMKb	PCR resultc		Clinical material
			Pc	Ctx		pbp2x	pbp2b
PSSP	1/H23	14	0.031	0.063	––––	+	−	Nasopharynx
	10/Z19	6	0.031	0.016	––––	−	−	CSF
PRSP
I	5/H31	6	2	0.5	–A––	+	+	Nasopharynx
	8/Z2	6	1	0.5	–A––	+	+	CSF
	11/Z20	6	1	0.5	–A––	+	+	CSF
	12/Z21	6	2	1	–A––	+	+	CSF
	22/HA5	6	0.25	0.25	––––	+	+	Phalynx
II	17/Z46	19	4	1	–A––	+	+	CSF
	20/B98	19	4	1	–A––	+	+	Blood
	27/SHA3	19	4	4	–A––	+	+	Sputum
III	18/B43	6	4	1	–S––	+	+	Blood
	19/B90	6	0.25	1	–S––	+	−	Blood
	28/H0	14	1	0.5	–S––	+	+	Nasopharynx
	2/H26	19	2	0.5	–S––	+	+	Nasopharynx
	3/H28	19	2	1	–S––	+	+	Nasopharynx
	6/KU126	19	2	1	–S––	+	+	Nasopharynx
	15/Z12	19	0.125	0.5	–S––	+	−	CSF
	16/Z13	19	4	1	–S––	+	+	CSF
	4/H29	23	2	0.5	–S––	+	+	Nasopharynx
	13/Z34	23	2	1	–S––	+	+	CSF
	21/B99	23	2	1	–S––	+	+	Blood
	7/KK133	23	2	4	–S––	+	+	Nasopharynx
IV	14/Z42	14	0.125	0.25	–S––	+	−	CSF
V	9/Z17	6	1	0.5	–S––	+	+	CSF
	23/HSB21	6	0.125	0.125	––––	+	+	Sputum
	24/TJ25	23	0.25	0.25	––––	+	+	Nasopharynx
	26/TJ29	23	0.25	0.25	––––	+	+	Nasopharynx

^aPc, penicillin; Ctx, cefotaxime. 

^bOnly amino acid residues differing from the STMK sequence at positions 370 to 373 in PBP 1A are shown. 

^cAltered PBP genes were detected by PCR as described previously (24). +, altered; −, not altered.