Figure 3.

Murine Igp5 specifically binds human amyloid deposits in formalin-fixed tissues. (A) Immunoreactivity of mIgp5 with human ATTRv, ALλ1, ALλ3, ALκ1, and ALECT2 amyloid as well as canine AA and murine hAIAPP in formalin-fixed, paraffin-embedded tissues. mIgp5 (brown DAB staining) co-localizes with amyloid deposits seen as green-gold birefringent material in Congo red-stained consecutive tissue sections. (B) Murine Igp5G does not bind human ATTRv or AL amyloid in amyloid-laden tissue sections, evidenced by the lack of brown staining. No binding of the mIgp5G was seen on normal human heart indicating the lack of reactivity of the m11-1F4 component with healthy cardiac tissue. Original objective magnification 20x.