Fig 5.

Selection of candidate macrophage invasion genes in chronic CF respiratory infections in vivo. QQ-plots of -log₁₀ transformed P-values testing the proportion of disruptive versus synonymous mutations in candidate genes Tn-Seq identified as having significant effects on macrophage invasion for both ATCC29213 and JE2 and corresponding control genes not implicated in that phenotype. P-values in ranges consistent with positive (more disruptive than synonymous mutations) and purifying selection (more synonymous than disruptive mutations) are indicated by the arrows at right. Significance of difference between the distributions of candidate and control genes by Wilcoxon rank-sum test is shown.